Human sTNFsR II ELISA Kit PicoKine¬Æ | Boster EK0530 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human sTNFsR II ELISA Kit PicoKine¬Æ

catalog :

EK0530

more info or order :

Boster product webpage

citations: 1

Reference
Ghallab N, El Wakeel N, Shaker O. Levels of salivary IFN-gamma, TNF-alfa, and TNF receptor-2 as prognostic markers in (erosive) oral lichen planus. Mediators Inflamm. 2010;2010:847632 pubmed publisher

image

image 1 :

Human sTNFsR II PicoKine ELISA Kit standard curve

product information

sku :

EK0530

status :

name :

Human sTNFsR II ELISA Kit PicoKine¬Æ

url key :

human-stnfsr-ii-picokine-trade-elisa-kit-ek0530-boster

product category :

Picokine ELISA Kits

gene name :

TNFRSF1B

price :

369

assay range :

7.8 pg/ml - 500 pg/ml

description :

Human sTNFsR II ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml.

short description :

Human sTNFsR II ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P20333

immunogen :

Expression system for standard: NS0; Immunogen sequence: L23-D257

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0530_2.png

image labels :

Human sTNFsR II PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.037 0.094 0.145 0.227 0.532 0.826 1.310 2.021

created at :

7/16/14 13:17

updated at :

3/2/22 2:54

meta title :

Human sTNFsR II ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human sTNFsR II ELISA Kit, TNF Receptor II ELISA Kit

meta description :

Human sTNFsR II ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml.

rating value :

100

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml, 7.8 pg/ml

background :

Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II (TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

research category :

Apoptosis,Associated Proteins,Cancer,Cell Biology,Cell Death,Growth Factors/Hormones,Receptors,Signal Transduction

synonyms :

Tumor necrosis factor receptor superfamily member 1B;Tumor necrosis factor receptor 2;TNF-R2;Tumor necrosis factor receptor type II;TNF-RII;TNFR-II;p75;p80 TNF-alpha receptor;CD120b;Etanercept;Tumor necrosis factor receptor superfamily member 1b, membrane form;Tumor necrosis factor-binding protein 2;TBP-2;TBPII;TNFRSF1B;TNFBR, TNFR2;

gene full name :

Tumor necrosis factor receptor superfamily member 1B

molecular weight :

48291 MW

protein function :

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

subcellular localization :

Isoform 1: Cell membrane; Single-pass type I membrane protein.

protein name :

Tumor necrosis factor receptor superfamily member 1B

sequence similarities :

Contains 4 TNFR-Cys repeats.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 17 16 16 0.43 2.6% Sample 2 58 61 58 56 58 1.78 3% Sample 3 235 274 227 275 252 21.93 8.7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

21403886

template :

elisa-kits

more info or order :

Boster product webpage