Human TNFsR I ELISA Kit PicoKine¬Æ | Boster EK0528 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TNFsR I ELISA Kit PicoKine¬Æ

catalog :

EK0528

more info or order :

Boster product webpage

citations: 1

Reference
Ataseven A, Kesli R, Kurtipek G, Ozturk P. Assessment of lipocalin 2, clusterin, soluble tumor necrosis factor receptor-1, interleukin-6, homocysteine, and uric acid levels in patients with psoriasis. Dis Markers. 2014;2014:541709 pubmed publisher

image

image 1 :

Human TNFsR I PicoKine ELISA Kit Standard Curve

product information

sku :

EK0528

status :

name :

Human TNFsR I ELISA Kit PicoKine¬Æ

url key :

human-tnfsr-i-picokine-trade-elisa-kit-ek0528-boster

product category :

Picokine ELISA Kits

gene name :

TNFRSF1A

price :

400

assay range :

7.8 pg/ml - 500 pg/ml

description :

Human TNFsR I ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

short description :

Human TNFsR I ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P19438

immunogen :

Expression system for standard: NS0; Immunogen sequence: L30-T211

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

application details :

Dilution ratio of 1:20, concentration in serum is around 3.2ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0528_1_1.png

image labels :

Human TNFsR I PicoKine ELISA Kit Standard Curve

sample data :

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.010 0.072 0.176 0.348 0.576 1.135 1.829 2.326

created at :

5/18/14 20:17

updated at :

3/2/22 2:54

meta title :

Human TNFsR I ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TNFsR I ELISA Kit, p65/TNF Receptor I ELISA

meta description :

Human TNFsR I ELISA Kit PicoKine (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

rating value :

100

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml, 7.8 pg/ml

background :

TNFsRI (also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF). In contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A), CD40, and FAS (TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.

research category :

Antiviral Signaling,Apoptosis,Associated Proteins,Cancer,Cell Biology,Cell Death,Growth Factors/Hormones,Immune System Diseases,Immunology,Receptors,Signal Transduction

synonyms :

Tumor necrosis factor receptor superfamily member 1A;Tumor necrosis factor receptor 1;TNF-R1;Tumor necrosis factor receptor type I;TNF-RI;TNFR-I;p55;p60;CD120a;Tumor necrosis factor receptor superfamily member 1A, membrane form;Tumor necrosis factor-binding protein 1;TBPI;TNFRSF1A;TNFAR, TNFR1;

gene full name :

Tumor necrosis factor receptor superfamily member 1A

molecular weight :

50495 MW

protein function :

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

subcellular localization :

Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single- pass type I membrane protein . Secreted . A secreted form is produced through proteolytic processing.

protein name :

Tumor necrosis factor receptor superfamily member 1A

sequence similarities :

Contains 1 death domain.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 10 54 219 10 59 203 Standard deviation 0.55 3.99 13.14 0.58 4.48 13.39 CV (%) 5.5% 7.4% 6% 5.8% 7.6% 6.6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 10 10 9 9 9 0.5 5.5% Sample 2 54 52 56 55 54 1.47 2.7% Sample 3 219 194 207 198 204 9.6 4.7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24803721

template :

elisa-kits

more info or order :

Boster product webpage