Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

catalog :

EK0527

more info or order :

Boster product webpage

citations: 3

Reference
Wang X, Yang J, Lu T, Zhan Z, Wei W, Lyu X, et al. The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression. Aging (Albany NY). 2020;12:11085-11099 pubmed publisher
Dong Y, Han Y, Wang Z, Qin Z, Yang C, Cao J, et al. Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice. BMC Gastroenterol. 2017;17:82 pubmed publisher
Werawatganon D, Rakananurak N, Sallapant S, Prueksapanich P, Somanawat K, Klaikeaw N, et al. Aloe vera attenuated gastric injury on indomethacin-induced gastropathy in rats. World J Gastroenterol. 2014;20:18330-7 pubmed publisher

image

image 1 :

Mouse TNF alpha PicoKine ELISA Kit standard curve

product information

sku :

EK0527

status :

name :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

url key :

mouse-tnf-alpha-picokine-trade-elisa-kit-ek0527-boster

product category :

Picokine ELISA Kits

gene name :

TNF

price :

369

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

short description :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P06804

immunogen :

Expression system for standard: E.coli; Immunogen sequence: L80-L235

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0527.png

image labels :

Mouse TNF alpha PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.090 0.156 0.281 0.443 0.723 1.284 1.958

created at :

5/18/14 20:17

updated at :

3/2/22 2:54

meta title :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine¬Æ

meta keyword :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity

meta description :

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

rating value :

99.2

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

background :

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

research category :

Metabolism,Signal Transduction,Immunology,Cancer,Atherosclerosis,Cardiovascular,Growth Factors/Hormones,Growth Factors,Innate Immunity,Vascular Inflammation,Cytokines,Tnf Superfamily

synonyms :

Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;

gene full name :

Tumor necrosis factor

molecular weight :

25896 MW

protein function :

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

subcellular localization :

Cell membrane; Single-pass type II membrane protein.

protein name :

Tumor necrosis factor

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from hamster polyclonal antibody from goat

normal range citations :

25561799

template :

elisa-kits

more info or order :

Boster product webpage