Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

catalog :

EK0526

more info or order :

Boster product webpage

citations: 1

Reference
Singh P, Castillo A, Majid D. Decrease in IL-10 and increase in TNF-? levels in renal tissues during systemic inhibition of nitric oxide in anesthetized mice. Physiol Rep. 2014;2:e00228 pubmed publisher

image

image 1 :

Rat TNF alpha PicoKine ELISA Kit standard curve

product information

sku :

EK0526

status :

name :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine¬Æ

url key :

rat-tnf-alpha-picokine-trade-elisa-kit-ek0526-boster

product category :

Picokine ELISA Kits

gene name :

TNF

price :

369

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Rat Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

short description :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Rat Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P16599

immunogen :

Expression system for standard: E.coli; Immunogen sequence: L80-L235

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0526.png

image labels :

Rat TNF alpha PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.031 0.062 0.132 0.203 0.555 0.884 1.838

created at :

5/19/14 3:17

updated at :

3/2/22 2:54

meta title :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine¬Æ

meta keyword :

Rat TNF Alpha/Tumor necrosis factor ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity

rating value :

99.6

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

background :

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

research category :

Metabolism,Signal Transduction,Immunology,Cancer,Atherosclerosis,Cardiovascular,Growth Factors/Hormones,Growth Factors,Innate Immunity,Vascular Inflammation,Cytokines,Tnf Superfamily

synonyms :

Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;

gene full name :

Tumor necrosis factor

molecular weight :

25806 MW

protein function :

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

subcellular localization :

Cell membrane ; Single-pass type II membrane protein .

protein name :

Tumor necrosis factor

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 96 280 13 98 306 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 14 14 14 14 0 0% Sample 2 96 93 92 101 95 3.5 3.6% Sample 3 280 258 274 306 279 17.28 6.1% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24744897

template :

elisa-kits

more info or order :

Boster product webpage