ELISA/assay

Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

EK0525

1

Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

human-tnf-alpha-picokine-trade-elisa-kit-ek0525-boster

Picokine ELISA Kits

TNF

369

15.6 pg/ml - 1,000 pg/ml

Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

96 wells/kit, with removable strips.

P01375

Expression system for standard: E.coli; Immunogen sequence: V77-L233

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

10 ng/tube

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Dilution ratio of 1:1, concentration in serum is around 100pg/ml

ELISA

Human

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Human TNF alpha PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.091 0.158 0.197 0.320 0.548 0.897 1.576 2.047

5/19/14 3:17

3/2/22 2:54

Human TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine®

Human TNF alpha ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity

97.6

heparin, EDTA or citrate

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Atherosclerosis,Cancer,Cardiovascular,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Metabolism,Signal Transduction,Tnf Superfamily,Vascular Inflammation

Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;TNF;TNFA, TNFSF2;

Tumor necrosis factor

25644 MW

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).

Cell membrane ; Single-pass type II membrane protein .

Tumor necrosis factor

Belongs to the tumor necrosis factor family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 82 201 14 75 186 Standard deviation 0.76 5.49 12.66 0.75 5.55 11.9 CV (%) 4.8% 6.7% 7.4% 5.4% 7.4% 6.4%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 15 16 15 0.5 3.3% Sample 2 82 91 75 89 84 6.29 7.4% Sample 3 201 204 215 219 209 7.46 3.5% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

24812899

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