Human TIMP-4 ELISA Kit PicoKine¬Æ | Boster EK0524 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TIMP-4 ELISA Kit PicoKine¬Æ

catalog :

EK0524

more info or order :

Boster product webpage

citations: 1

Reference
Papadopoulos D, Thomopoulos C, Perrea D, Papazachou U, Daskalaki M, Bourantas C, et al. Changes of metalloproteinases profile in patients with masked hypertension. Angiology. 2009;60:757-62 pubmed publisher

image

image 1 :

Human TIMP-4 PicoKine ELISA Kit standard curve

product information

sku :

EK0524

status :

name :

Human TIMP-4 ELISA Kit PicoKine¬Æ

url key :

human-timp-4-picokine-trade-elisa-kit-ek0524-boster

product category :

Picokine ELISA Kits

gene name :

TIMP4

price :

369

assay range :

78 pg/ml - 5,000 pg/ml

description :

Human TIMP-4 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

short description :

Human TIMP-4 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q99727

immunogen :

Expression system for standard: sf21; Immunogen sequence: C30-P224

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

application details :

Dilution ratio of 1:1, concentration in serum is around 1000pg/ml

applications :

ELISA

reactivity :

Human

images :

/antibody/ek0524-2-ELISA-human-timp-4-picokine-elisa-kit.jpg

image labels :

Human TIMP-4 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.006 0.109 0.232 0.519 0.998 1.381 1.809 2.166

created at :

7/17/14 3:17

updated at :

3/2/22 2:54

meta title :

Human TIMP-4 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TIMP-4 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human TIMP-4 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

rating value :

98.9

anticoagulant :

heparin or EDTA

assay procedure step 1 :

5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml, 78 pg/ml

background :

Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

research category :

Angiogenesis,Cancer,Cardiovascular,Cell Biology,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis / Ubiquitin

synonyms :

Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;TIMP4;

gene full name :

Metalloproteinase inhibitor 4

molecular weight :

25503 MW

protein function :

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9.

subcellular localization :

Secreted.

tissue specificity :

Abundant in heart and present at low levels in many other tissues.

protein name :

Metalloproteinase inhibitor 4

sequence similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 131 119 122 123 4.43 3.6% Sample 2 589 618 605 599 602 10.49 1.7% Sample 3 2056 2211 2029 2343 2159 126.55 5.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

19114409

template :

elisa-kits

more info or order :

Boster product webpage