ELISA/assay

Human TIMP-3 ELISA Kit PicoKine®

EK0523

1

Human TIMP-3 ELISA Kit PicoKine®

human-timp-3-picokine-trade-elisa-kit-ek0523-boster

Picokine ELISA Kits

TIMP3

400

156 pg/ml - 10,000 pg/ml

Human TIMP-3 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Human TIMP-3 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

96 wells/kit, with removable strips.

P35625

Expression system for standard: NS0; Immunogen sequence: C24-P211

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<2 pg/ml

10 ng/tube

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Dilution ratio of 1:1, concentration in serum is 600-1200pg/ml

ELISA

Human

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Human TIMP-3 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.079 0.151 0.173 0.250 0.433 0.746 1.459 2.068

5/19/14 10:17

3/2/22 2:54

Human TIMP-3 ELISA Kit Price PicoKine® BosterBio

Human TIMP-3 ELISA Kit PicoKine, Bosterbio High Sensitivity

Human TIMP-3 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

98.8

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Angiogenesis,Apoptosis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Neuroscience,Protease Inhibitors,Proteolysis / Ubiquitin,Sensory System,Signal Transduction,Visual System

Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3;

Metalloproteinase inhibitor 3

24145 MW

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Secreted, extracellular space, extracellular matrix.

Metalloproteinase inhibitor 3

Belongs to the protease inhibitor I35 (TIMP) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

25449074

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