Human TIMP-2 ELISA Kit PicoKine¬Æ | Boster EK0522 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TIMP-2 ELISA Kit PicoKine¬Æ

catalog :

EK0522

more info or order :

Boster product webpage

citations: 1

Reference
Di Carlo A. Matrix metalloproteinase-2 and -9 and tissue inhibitor of metalloproteinase-1 and -2 in sera and urine of patients with renal carcinoma. Oncol Lett. 2014;7:621-626 pubmed

image

image 1 :

Human TIMP-2 PicoKine ELISA Kit standard curve

product information

sku :

EK0522

status :

name :

Human TIMP-2 ELISA Kit PicoKine¬Æ

url key :

human-timp-2-picokine-trade-elisa-kit-ek0522-boster

product category :

Picokine ELISA Kits

gene name :

TIMP2

price :

380

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human TIMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

short description :

Human TIMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P16035

immunogen :

Expression system for standard: CHO; Immunogen sequence: C27-P220

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0522.png

image labels :

Human TIMP-2 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909

created at :

7/17/14 10:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human TIMP-2 PicoKine Fast ELISA Kit and many others.

meta title :

Human TIMP-2 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TIMP-2 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human TIMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

research category :

Angiogenesis,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis / Ubiquitin,Signal Transduction

synonyms :

Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2;

gene full name :

Metalloproteinase inhibitor 2

molecular weight :

24399 MW

protein function :

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

subcellular localization :

Secreted.

protein name :

Metalloproteinase inhibitor 2

sequence similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24520285

template :

elisa-kits

more info or order :

Boster product webpage