Mouse TIMP-1 ELISA Kit PicoKine¬Æ | Boster EK0521 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse TIMP-1 ELISA Kit PicoKine¬Æ

catalog :

EK0521

more info or order :

Boster product webpage

citations: 1

Reference
Kim E, Choi M, Park H, Kim M, Hong J, Lee J, et al. Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice. Breast Cancer Res. 2011;13:R78 pubmed publisher

image

image 1 :

Mouse TIMP-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0521

status :

name :

Mouse TIMP-1 ELISA Kit PicoKine¬Æ

url key :

mouse-timp-1-picokine-trade-elisa-kit-ek0521-boster

product category :

Picokine ELISA Kits

gene name :

TIMP1

price :

369

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Mouse TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Mouse TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P12032

immunogen :

Expression system for standard: NS0; Immunogen sequence: C25-R205

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0521_2.png

image labels :

Mouse TIMP-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.118 0.138 0.229 0.413 0.709 1.431 2.084

created at :

10/23/14 19:49

updated at :

3/2/22 2:55

meta title :

Mouse TIMP-1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Mouse TIMP-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Mouse TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What‚Äôs more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.

research category :

Angiogenesis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis / Ubiquitin,Signal Transduction

synonyms :

Metalloproteinase inhibitor 1;Collagenase inhibitor 16C8 fibroblast;Erythroid-potentiating activity;EPA;TPA-S1;TPA-induced protein;Tissue inhibitor of metalloproteinases 1;TIMP-1;Timp1;Timp, Timp-1;

gene full name :

Metalloproteinase inhibitor 1

molecular weight :

22628 MW

protein function :

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

subcellular localization :

Secreted .

tissue specificity :

Found in fetal and adult tissues. Highest levels are found in bone. Also found in lung, ovary and uterus.

protein name :

Metalloproteinase inhibitor 1

sequence similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 258 787 62 246 710 Standard deviation 3.44 16.51 41.71 4.21 20.41 45.44 CV (%) 5.3% 6.4% 5.3% 6.8% 8.3% 6.4%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 74 72 73 71 3.53 4.9% Sample 2 258 273 282 277 272 8.95 3.2% Sample 3 787 883 759 760 797 50.76 6.3% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

normal range citations :

21834963

template :

elisa-kits

more info or order :

Boster product webpage