Human TIMP-1 ELISA Kit PicoKine¬Æ | Boster EK0520 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TIMP-1 ELISA Kit PicoKine¬Æ

catalog :

EK0520

more info or order :

Boster product webpage

citations: 1

Reference
Dhir V, Srivastava R, Aggarwal A. Circulating Levels of Soluble Receptor Activator of NF- κ B Ligand and Matrix Metalloproteinase 3 (and Their Antagonists) in Asian Indian Patients with Ankylosing Spondylitis. Int J Rheumatol. 2013;2013:814350 pubmed publisher

image

image 1 :

Human TIMP-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0520

status :

name :

Human TIMP-1 ELISA Kit PicoKine¬Æ

url key :

human-timp-1-picokine-trade-elisa-kit-ek0520-boster

product category :

Picokine ELISA Kits

gene name :

TIMP1

price :

369

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Human TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 5pg/ml.

short description :

Human TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 5pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P01033

immunogen :

Expression system for standard: NS0; Immunogen sequence: C24-A207

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

application details :

Dilution ratio of 1:100, concentration in serum is around 150ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0520_1_2.png

image labels :

Human TIMP-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.438 0.759 1.197 1.322 1.669 1.805 2.033

created at :

7/17/14 10:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human TIMP-1 PicoKine Fast ELISA Kit and many others.

meta title :

Human TIMP-1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TIMP-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 5pg/ml.

rating value :

99.4

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

background :

The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.

research category :

Angiogenesis,Cancer,Cardiovascular,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis / Ubiquitin,Signal Transduction

synonyms :

Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1;TIMP-1;TIMP1;CLGI, TIMP;

gene full name :

Metalloproteinase inhibitor 1

molecular weight :

23171 MW

protein function :

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

subcellular localization :

Secreted .

tissue specificity :

Detected in rheumatoid synovial fluid (at protein level).

protein name :

Metalloproteinase inhibitor 1

sequence similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 194 429 28 209 433 Standard deviation 1.59 12.22 29.17 2.12 14.63 36.37 CV (%) 6.8% 7% 6.8% 7.6% 7% 8.4%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 31 30 30 30 0.7 2.3% Sample 2 155 156 165 162 159 4.15 2.6% Sample 3 411 409 455 402 419 20.9 4.9% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24078814

template :

elisa-kits

more info or order :

Boster product webpage