Rabbit TGF Alpha ELISA Kit PicoKine® | Boster EK0511-RB product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rabbit TGF Alpha ELISA Kit PicoKine®

catalog :

EK0511-RB

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0511-RB

Product Name :

Rabbit TGF Alpha ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Rabbit TGF Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Rabbit TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and rabbit milk. Sensitivity: 1pg/ml.

Reactivity :

Rabbit

Application :

ELISA

Gene Name :

TGFA

Uniprot ID :

P01135

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Cross-reactivity :

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and rabbit milk

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Protransforming growth factor alpha

Synonyms :

Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA;

Protein Name :

Protransforming growth factor alpha

Molecular Weight :

17006 MW

Protein Function :

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Subcellular Localization :

Transforming growth factor alpha: Secreted, extracellular space.

Tissue Specificity :

Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.

Background :

Transforming growth factor alpha (TGF-alpha) is upregulated in some rabbit cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to rabbit chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic rabbit TGF-alpha showed similar immunoreactivity when compared with rabbit TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction