Boster

ELISA/assay

Human MMP13 PicoKine ELISA Kit

EK0468

96 wells/kit

400 USD

EK0468

Human MMP13 PicoKine ELISA Kit

400 USD

Expression system for standard: NSO; Immunogen sequence: L20-C471

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP13

96 wells/kit

Human

ELISA

Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

There is no detectable cross-reactivity with other relevant proteins.

Collagenase 3

MMP13

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Collagenase 3

cardiovascular angiogenesis adhesion / ecm matrix metalloproteinases mmp signal transduction cytoskeleton / ecm extracellular matrix ecm enzymes cancer invasion/microenvironment mmps cell biology proteolysis / ubiquitin proteolytic enzymes metalloprotease tumor biomarkers kits/ lysates/ other elisa kits tumor biomarkers elisa kits

Belongs to the peptidase M10A family.

Store at 4ËšC for 6 months, at -20ËšC for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Secreted, extracellular space, extracellular matrix . Secreted .

Collagenase 3;3.4.24.-;Matrix metalloproteinase-13;MMP-13;MMP13;

Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.

A8K846