Boster

ELISA/assay

Mouse MMP-2 PicoKine ELISA Kit

EK0460

96 wells/kit

400 USD

EK0460

Mouse MMP-2 PicoKine ELISA Kit

400 USD

Expression system for standard: NSO; Immunogen sequence: A30-C662

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-2

96 wells/kit

Mouse

ELISA

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

There is no detectable cross-reactivity with other relevant proteins.

72 kDa type IV collagenase

MMP2; Mmp2

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).

72 kDa type IV collagenase

cardiovascular angiogenesis adhesion / ecm matrix metalloproteinases mmp cancer invasion/microenvironment extracellular matrix mmps cell biology proteolysis / ubiquitin proteolytic enzymes metalloprotease tumor biomarkers cancer metabolism response to hypoxia metabolism pathways and processes metabolism processes types of disease

Belongs to the peptidase M10A family.

Store at 4ËšC for 6 months, at -20ËšC for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Isoform 1: Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).

72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;PEX;Mmp2;

P33434