Human MMP-1 PicoKine ELISA Kit | Boster EK0458 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MMP-1 PicoKine ELISA Kit

catalog :

EK0458

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
Deng C, Ling S, Liu X, Zhao Y, Lv Y. Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia. Exp Ther Med. 2015;9:992-998 pubmed

image

image 1 :

Human MMP-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0458

status :

Enabled

name :

Human MMP-1 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

MMP1

price :

380 USD

assay range :

156pg/ml-10000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human MMP1 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP1 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-1. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

B4DN15

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: F20-N469

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<20pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin).

applications :

ELISA

reactivity :

Human

image labels :

Human MMP-1 PicoKine ELISA Kit standard curve

anticoagulant :

heparin

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml

background :

Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

research category :

Signal Transduction, Tumor Biomarkers

synonyms :

cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7) ;

gene full name :

matrix metallopeptidase 1 (interstitial collagenase)

molecular weight :

46266 MW

protein name :

Interstitial collagenase

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV(%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.096 0.130 0.219 0.389 0.661 1.160 2.096

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/26/19 1:53

more info or order :

Boster product webpage