Human CCL19/MIP-3 Beta PicoKine ELISA Kit | Boster EK0456 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CCL19/MIP-3 Beta PicoKine ELISA Kit

catalog :

EK0456

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Ellingsen T, Hansen I, Thorsen J, Møller B, Tarp U, Lottenburger T, et al. Upregulated baseline plasma CCL19 and CCR7 cell-surface expression on monocytes in early rheumatoid arthritis normalized during treatment and CCL19 correlated with radiographic progression. Scand J Rheumatol. 2014;43:91-100 pubmed publisher

image

image 1 :

Human CCL19/MIP-3 beta PicoKine ELISA Kit standard curve

product information

sku :

EK0456

status :

Enabled

name :

Human CCL19/MIP-3 Beta PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CCL19

price :

369 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Mip-3 Beta/CCL19 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip-3 Beta/CCL19 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL19/MIP-3 beta. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

Q6IBD6

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: G22-S98

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human CCL19/MIP-3 beta PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Chemokine(C-C motif) ligand 19(CCL19) is a small cytokineÂ belonging to the CC chemokine family that is also known asÂ EBI1 ligand chemokine(ELC) and macrophage inflammatory protein-3-betaÂ (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

research category :

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

synonyms :

C-C motif chemokine ;CCL19 ;hCG_20476 ;

gene full name :

C-C motif chemokine

molecular weight :

10993 MW

subcellular localization :

Secreted .

protein name :

C-C motif chemokine 19

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 CV(%) 4.8% 6.7% 6.4% 5% 7.5% 8.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 30 31 29 29 0.82 2.8% Sample 2 234 255 249 222 240 12.9 5.3% Sample 3 421 456 425 465 441 19.07 4.3% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.066 0.128 0.191 0.395 0.770 1.506 2.265

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage