Rat MIP-3 Alpha/CCL20 PicoKine ELISA Kit | Boster EK0455 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat MIP-3 Alpha/CCL20 PicoKine ELISA Kit

catalog :

EK0455

quantity :

96 wells/kit, with removable strips.

price :

390 USD

more info or order :

Boster product webpage

citations: 1

Reference
Das M, Leonardo C, Rangooni S, Pennypacker K, Mohapatra S, Mohapatra S. Lateral fluid percussion injury of the brain induces CCL20 inflammatory chemokine expression in rats. J Neuroinflammation. 2011;8:148 pubmed publisher

product information

sku :

EK0455

status :

Enabled

name :

Rat MIP-3 Alpha/CCL20 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CCL20

price :

390 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Rat Mip3A/CCL20 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip3A/CCL20 tissue specificity: Low levels in thymus and lung.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MIP-3 alpha/CCL20. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P97884

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: S27-M96

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Rat

image labels :

Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

research category :

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

synonyms :

C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;CC chemokine ST38;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;Ccl20;Scya20, St38;

gene full name :

C-C motif chemokine 20

molecular weight :

10875 MW

protein function :

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (By similarity). May play a role in modulating inflammatory cell recruitment to the CNS and therefore contribute to tissue injury in ischemic stroke and autoimmune diseases.

subcellular localization :

Secreted.

tissue specificity :

Low levels in thymus and lung.

protein name :

C-C motif chemokine 20

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV(%) 5.2% 5% 5.6% 5.9% 5.8% 6.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 88 98 96 93 3.76 4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 3 782 809 756 768 778 19.74 2.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000 O.D. 0.027 0.126 0.145 0.240 0.313 0.497 1.188 2.014

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage