Mouse MIP-3 Alpha/CCL20 PicoKine ELISA Kit | Boster EK0454 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse MIP-3 Alpha/CCL20 PicoKine ELISA Kit

catalog :

EK0454

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, et al. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE. 2014;9:e93057 pubmed publisher

image

image 1 :

Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

product information

sku :

EK0454

status :

Enabled

name :

Mouse MIP-3 Alpha/CCL20 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CCL20

price :

369 USD

assay range :

7.8pg/ml-500pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse Mip3A/CCL20 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip3A/CCL20 tissue specificity: Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-3 alpha/CCL20. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

O89093

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A28-M97

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

background :

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

research category :

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

synonyms :

C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;CC chemokine ST38;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;Ccl20;Larc, Scya20;

gene full name :

C-C motif chemokine 20

molecular weight :

10826 MW

protein function :

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.

subcellular localization :

Secreted.

tissue specificity :

Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

protein name :

C-C motif chemokine 20

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 9 55 313 9 57 308 Standard deviation 0.62 3.02 21.28 0.78 3.7 21.56 CV(%) 6.9% 5.5% 6.8% 8.7% 6.5% 7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 9 10 10 9 0.5 5.5% Sample 2 55 56 52 51 53 2.06 3.8% Sample 3 313 301 271 296 295 15.3 5.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.023 0.201 0.394 0.761 1.251 1.852 1.996 2.090

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage