Human MIP-3 Alpha / CCL20 / MIP3a PicoKine ELISA Kit | Boster EK0453 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MIP-3 Alpha / CCL20 / MIP3a PicoKine ELISA Kit

catalog :

EK0453

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
Chang K, Hao S, Chang J, Wu C, Tsang N, Lee Y, et al. Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes. Clin Cancer Res. 2008;14:6979-87 pubmed publisher

image

image 1 :

Human MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

product information

sku :

EK0453

status :

Enabled

name :

Human MIP-3 Alpha / CCL20 / MIP3a PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CCL20

price :

380 USD

assay range :

7.8pg/ml-500pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Mip3A/CCL20 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip3A/CCL20 tissue specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIP-3 alpha. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P78556

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A27-M96

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

background :

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

research category :

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

synonyms :

C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;CCL20(1-67);CCL20(1-64);CCL20(2-70);CCL20;LARC, MIP3A, SCYA20;

gene full name :

C-C motif chemokine 20

molecular weight :

10762 MW

protein function :

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.

subcellular localization :

Secreted.

tissue specificity :

Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

protein name :

C-C motif chemokine 20

sequence similarities :

Belongs to the intercrine beta (chemokine CC) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 19 78 256 17 73 269 Standard deviation 1.34 4.91 13.05 1.22 5.03 15.06 CV(%) 7.1% 6.9% 5.6% 7.2% 6.9% 5.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 18 19 19 18 0.43 2.3% Sample 2 78 79 72 74 75 2.86 3.8% Sample 3 256 262 263 263 261 2.91 1.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.053 0.105 0.171 0.306 0.478 0.992 1.467 2.144

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage