Mouse MIP-2 PicoKine ELISA Kit | Boster EK0452 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse MIP-2 PicoKine ELISA Kit

catalog :

EK0452

quantity :

96 wells/kit, with removable strips.

price :

420 USD

more info or order :

Boster product webpage

citations: 1

Reference
Lee B, Lee T, Jung J, Oh D, Choi J, Shin J, et al. The role of keratinocyte-derived chemokine in hemorrhage-induced acute lung injury in mice. J Korean Med Sci. 2009;24:775-81 pubmed publisher

image

image 1 :

Mouse MIP-2 PicoKine ELISA Kit standard curve

product information

sku :

EK0452

status :

Enabled

name :

Mouse MIP-2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CXCL2

price :

420 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse Mip-2/CXCL2 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip-2/CXCL2 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P10889

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A28-N100

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse MIP-2 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein(MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein(MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water(aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa.

research category :

Chemokines, Immunology, Innate Immunity

synonyms :

C-X-C motif chemokine 2;Macrophage inflammatory protein 2;MIP2;Cxcl2;Mip-2, Mip2, Scyb2;

gene full name :

C-X-C motif chemokine 2

molecular weight :

10621 MW

protein function :

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

subcellular localization :

Secreted.

protein name :

C-X-C motif chemokine 2

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 39 230 434 37 220 409 Standard deviation 1.79 17.94 17.79 1.85 18.7 22.9 CV(%) 4.6% 7.8% 4.1% 5% 8.5% 5.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 34 40 41 38 2.69 7% Sample 2 230 234 204 215 220 11.98 5.4% Sample 3 434 404 419 468 431 23.72 5.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.130 0.249 0.425 0.811 1.227 1.833 2.039

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage