Human MIP-1Alpha/CCL3 PicoKine ELISA Kit | Boster EK0448 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MIP-1Alpha/CCL3 PicoKine ELISA Kit

catalog :

EK0448

quantity :

96 wells/kit, with removable strips.

price :

350 USD

more info or order :

Boster product webpage

citations: 1

Reference
Zhang J, Gao J. [Measurement of MIP-1alpha and TNF-alpha in seminal plasma and its clinical significance]. Zhonghua Nan Ke Xue. 2012;18:627-30 pubmed

image

image 1 :

Human MIP-1 alpha PicoKine ELISA Kit standard curve

product information

sku :

EK0448

status :

Enabled

name :

Human MIP-1Alpha/CCL3 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

CCL3

price :

350 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Mip 1Alpha/CCL3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1Alpha/CCL3 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIP-1 alpha. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

A0N0R1

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A27-A92

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human MIP-1 alpha PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.

research category :

Macrophage / Inflamm.

synonyms :

C-C motif chemokine ;CCL3 ;hCG_1749202 ;

gene full name :

C-C motif chemokine

molecular weight :

10085 MW

subcellular localization :

Secreted .

protein name :

C-C motif chemokine 3

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 33 175 370 30 166 361 Standard deviation 2.54 10.32 14.8 2.58 12.45 20.93 CV(%) 7.7% 4% 4% 8.6% 7.5% 5.8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 35 33 1.11 3.3% Sample 2 175 166 172 161 168 5.4 3.2% Sample 3 370 407 390 394 390 13.27 3.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.037 0.112 0.206 0.272 0.545 0.932 1.456 2.071

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

4/10/19 2:01

more info or order :

Boster product webpage