Mouse CCL22/MDC PicoKine ELISA Kit | Boster EK0447 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CCL22/MDC PicoKine ELISA Kit

catalog :

EK0447

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
Yin K, Jin S, Bian T, Wu Q, Wang X, Yao X. [The effects of imiquimod on an animal model of asthma]. Zhonghua Jie He He Hu Xi Za Zhi. 2007;30:509-17 pubmed

image

image 1 :

Mouse CCL22/MDC PicoKine ELISA Kit standard curve

product information

sku :

EK0447

status :

Enabled

name :

Mouse CCL22/MDC PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CCL22

price :

380 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse CCL22 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CCL22 tissue specificity: Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MDC. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

O88430

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: G25-S92

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse CCL22/MDC PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

research category :

Chemokines, Immunology, Innate Immunity

synonyms :

C-C motif chemokine 22;Activated B and dendritic cell-derived;CC chemokine ABCD-1;Small-inducible cytokine A22;Ccl22;Abcd1, Scya22;

gene full name :

C-C motif chemokine 22

molecular weight :

10302 MW

protein function :

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B- lymphocytes with T-cells in immune responses.

subcellular localization :

Secreted.

tissue specificity :

Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.

protein name :

C-C motif chemokine 22

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 46 233 820 52 308 871 Standard deviation 2.94 17.48 50.84 4.11 25.26 66.20 CV(%) 6.4% 7.5% 6.2% 7.9% 8.2% 7.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 54 48 49 3.03 6.1% Sample 2 233 248 234 235 237 6.10 2.5% Sample 3 820 852 936 892 875 43.48 4.9% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.035 0.046 0.086 0.186 0.500 1.146 2.111

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage