Human M-CSF PicoKine ELISA Kit | Boster EK0444 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human M-CSF PicoKine ELISA Kit

catalog :

EK0444

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Bahar B, Acedil Ayc Iota B, Coskun U, Buyukberber S, Benekli M, Yildiz R. Granulocyte colony stimulating factor (G-CSF) and macrophage colony stimulating factor (M-CSF) as potential tumor markers in non small cell lung cancer diagnosis. Asian Pac J Cancer Prev. 2010;11:709-12 pubmed

image

image 1 :

Human M-CSF PicoKine ELISA Kit standard curve

product information

sku :

EK0444

status :

Enabled

name :

Human M-CSF PicoKine ELISA Kit

category name :

Cytokine Network

gene name :

CSF1

price :

320 USD

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human M-Csf/CSF1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. M-Csf/CSF1 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human M-CSF. 96wells/kit, with removable strips.

description1 :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human M-CSF PicoKine ELISA Kit and many others.

size :

96 wells/kit, with removable strips.

uniprot id :

P09603

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: E33-S190

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine.

applications :

ELISA

reactivity :

Human

image labels :

Human M-CSF PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.

research category :

Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation

synonyms :

Macrophage colony-stimulating factor 1;CSF-1;M-CSF;MCSF;Lanimostim;Processed macrophage colony-stimulating factor 1;CSF1;

gene full name :

Macrophage colony-stimulating factor 1

molecular weight :

60179 MW

protein function :

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

subcellular localization :

Cell membrane ; Single-pass type I membrane protein .

protein name :

Macrophage colony-stimulating factor 1

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV(%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 153 143 153 150 4.2 2.8% Sample 2 681 697 651 751 695 36.3 5.2% Sample 3 1139 1296 1284 1344 1265 76.54 6% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage