Human CCL7/MCP-3 PicoKine ELISA Kit | Boster EK0443 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CCL7/MCP-3 PicoKine ELISA Kit

catalog :

EK0443

quantity :

96 wells/kit, with removable strips.

price :

333 USD

more info or order :

Boster product webpage

citations: 2

Reference
Jung D, Che Z, Kim J, Kim K, Kim K, Williams D, et al. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int J Cancer. 2010;127:332-44 pubmed publisher
Yanaba K, Komura K, Kodera M, Matsushita T, Hasegawa M, Takehara K, et al. Serum levels of monocyte chemotactic protein-3/CCL7 are raised in patients with systemic sclerosis: association with extent of skin sclerosis and severity of pulmonary fibrosis. Ann Rheum Dis. 2006;65:124-6 pubmed

image

image 1 :

Human CCL7/MCP-3 PicoKine ELISA Kit standard curve

product information

sku :

EK0443

status :

Enabled

name :

Human CCL7/MCP-3 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

CCL7

price :

333 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Mcp3/CCL7 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mcp3/CCL7 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL7/MCP-3. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P80098

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: Q24-L99

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human CCL7/MCP-3 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

research category :

Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm.

synonyms :

C-C motif chemokine 7;Monocyte chemoattractant protein 3;Monocyte chemotactic protein 3;MCP-3;NC28;Small-inducible cytokine A7;CCL7;MCP3, SCYA6, SCYA7;

gene full name :

C-C motif chemokine 7

molecular weight :

11200 MW

protein function :

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

subcellular localization :

Secreted.

protein name :

C-C motif chemokine 7

sequence similarities :

Belongs to the intercrine beta (chemokine CC) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 38 169 505 37 158 504 Standard deviation 2.39 12.67 27.27 2.55 12.79 31.75 CV(%) 8.1% 7.5% 5.4% 6.9% 8.1% 6.3%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 31 31 37 34 3.26 9.5% Sample 2 169 170 168 164 167 2.27 1.3% Sample 3 505 504 427 423 464 39.77 8.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.182 0.323 0.550 1.003 1.418 1.877 2.179

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

5/7/19 5:43

more info or order :

Boster product webpage