Human MCP-1 / CCL2 PicoKine ELISA Kit | Boster EK0441 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MCP-1 / CCL2 PicoKine ELISA Kit

catalog :

EK0441

quantity :

96 wells/kit, with removable strips.

price :

300 USD

more info or order :

Boster product webpage

citations: 1

Reference
Abujam B, Cheekatla S, Aggarwal A. Urinary CXCL-10/IP-10 and MCP-1 as markers to assess activity of lupus nephritis. Lupus. 2013;22:614-23 pubmed publisher

image

image 1 :

Human MCP-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0441

status :

Enabled

name :

Human MCP-1 / CCL2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

CCL2

price :

300 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Mcp1/CCL2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mcp1/CCL2 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MCP-1. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P13500

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: Q24-T99

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is cross-reactivity with human Eotaxin, MCP-3<1%.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

applications :

ELISA

reactivity :

Human

image labels :

Human MCP-1 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

research category :

Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes, Response To Hypoxia, Vascular Inflammation

synonyms :

C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2;

gene full name :

C-C motif chemokine 2

molecular weight :

11025 MW

protein function :

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

subcellular localization :

Secreted.

protein name :

C-C motif chemokine 2

sequence similarities :

Belongs to the intercrine beta (chemokine CC) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV(%) 5% 5.5% 6.9% 6.6% 6.6% 7.8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.154 0.244 0.466 1.052 1.298 1.892 2.175

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

last modified :

3/14/19 17:14

more info or order :

Boster product webpage