Mouse Leptin Receptor PicoKine ELISA Kit | Boster EK0440 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Leptin Receptor PicoKine ELISA Kit

catalog :

EK0440

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
Hsuchou H, Jayaram B, Kastin A, Wang Y, Ouyang S, Pan W. Endothelial cell leptin receptor mutant mice have hyperleptinemia and reduced tissue uptake. J Cell Physiol. 2013;228:1610-6 pubmed publisher

image

image 1 :

Mouse Leptin receptor PicoKine ELISA Kit standard curve

product information

sku :

EK0440

status :

Enabled

name :

Mouse Leptin Receptor PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

LEPR

price :

380 USD

assay range :

156pg/ml-10,000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse Leptin R/LEPR with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Leptin R/LEPR tissue specificity: Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Leptin receptor. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P48356

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: L22-G839

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse Leptin receptor PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml

background :

Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

research category :

Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Signal Transduction, Structures

synonyms :

Leptin receptor;LEP-R;B219;OB receptor;OB-R;CD295;Lepr;Db, Obr;

gene full name :

Leptin receptor

molecular weight :

130789 MW

protein function :

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.

subcellular localization :

Cell membrane; Single-pass type I membrane protein.

tissue specificity :

Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.

protein name :

Leptin receptor

sequence similarities :

Belongs to the type I cytokine receptor family. Type 2 subfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 222 1398 4930 200 1476 5345 Standard deviation 15.09 86.8%7 359.89 15.8 110.7 427.6 CV(%) 6.8% 6.1% 7.3% 7.9% 7.5% 8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 189 209 209 207 11.79 5.6% Sample 2 1398 1396 1505 1381 1420 49.51 3.4% Sample 3 4930 5654 5332 5633 5387 293.12 5.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.016 0.070 0.128 0.248 0.458 0.817 1.452 2.111

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage