Human Leptin PicoKine ELISA Kit | Boster EK0437 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Leptin PicoKine ELISA Kit

catalog :

EK0437

quantity :

96 wells/kit, with removable strips.

price :

300 USD

more info or order :

Boster product webpage

citations: 1

Reference
Mozafari N, Robati R, Younespour S. Serum leptin levels in pemphigus: a case control study. Biomed Res Int. 2014;2014:853705 pubmed publisher

image

image 1 :

Human Leptin PicoKine ELISA Kit standard curve

product information

sku :

EK0437

status :

Enabled

name :

Human Leptin PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

LEP

price :

300 USD

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Leptin/LEP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Leptin/LEP tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Leptin. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

A4D0Y8

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: V22-C167

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human Leptin PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

research category :

Atherosclerosis, Cancer, Cardiovascular, Diabetes Associated, Growth Factors/Hormones, Heart Disease, Mesenchymal Stem Cells, Metabolism, Neurology Process, Neuroscience, Obesity, Signal Transduction, Stem Cells

synonyms :

Leptin ;Obesity factor ;LEP ;hCG_33000 , tcag7.84 ;

gene full name :

Leptin

molecular weight :

18641 MW

protein function :

May function as part of a signaling pathway that acts to regulate the size of the body fat depot.

subcellular localization :

Secreted .

protein name :

Leptin

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV(%) 7.6% 6.7% 5.2% 8.4% 8% 7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 83 84 87 83 2.16 2.6% Sample 2 485 539 463 476 490 28.93 5.9% Sample 3 1057 1101 1114 1116 1097 23.8 2.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.036 0.080 0.191 0.505 0.923 1.801 2.461

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage