Rat LAMA1 PicoKine ELISA Kit | Boster EK0435 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat LAMA1 PicoKine ELISA Kit

catalog :

EK0435

quantity :

96 wells/kit, with removable strips.

price :

260 USD

more info or order :

Boster product webpage

citations: 1

Reference
Qian Y, Fu X, Hu R, Shen L, Bai H. [Effects of Corbrin Shugan capsule on dimethylnitrosamine-induced hepatic fibrosis in rats]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2013;42:561-6 pubmed

image

image 1 :

Rat LAMA1 PicoKine ELISA Kit standard curve

product information

sku :

EK0435

status :

Enabled

name :

Rat LAMA1 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

LAMA1; LAMA2; LAMB1; LAMB2

price :

260 USD

assay range :

156pg/ml-10,000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Rat Laminin Alpha 1/LAMA1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Laminin Alpha 1/LAMA1 tissue specificity: Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat LAMA1. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P15800

host :

immunogen :

Expression system for standard: from rat sarcoma basement membrane

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Rat

image labels :

Rat LAMA1 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml

background :

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

research category :

Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Intermediate Filaments, Neuroscience, Signal Transduction

synonyms :

Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2;

gene full name :

Laminin subunit beta-2

molecular weight :

196474 MW

protein function :

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

subcellular localization :

Secreted, extracellular space, extracellular matrix, basement membrane.

tissue specificity :

Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.

protein name :

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2

sequence similarities :

Contains 13 laminin EGF-like domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 256 1435 4770 279 1524 4389 Standard deviation 11.26 97.58 352.98 15.34 128.01 373.06 CV(%) 4.4% 6.8% 7.4% 5.5% 8.4% 8.5%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 256 282 284 258 270 13.03 4.8% Sample 2 1435 1315 1498 1568 1454 93.02 6.3% Sample 3 4770 4867 4216 4900 4688 276.8 5.9% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.097 0.120 0.163 0.252 0.482 0.930 1.654 2.438

tmb incubation time :

15-25min

principle :

polyclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage