Human IL-17 PicoKine ELISA Kit | Boster EK0430 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-17 PicoKine ELISA Kit

catalog :

EK0430

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Alyasin S, Karimi M, Amin R, Babaei M, Darougar S. Interleukin-17 gene expression and serum levels in children with severe asthma. Iran J Immunol. 2013;10:177-85 pubmed publisher

image

image 1 :

Human IL-17 PicoKine ELISA Kit standard curve

product information

sku :

EK0430

status :

Enabled

name :

Human IL-17 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL17A

price :

320 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Il 17/IL17A with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 17/IL17A tissue specificity: Restricted to activated memory T-cells.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

Q16552

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: I20-A155

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-17 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage / Inflamm.

synonyms :

Interleukin-17A;IL-17;IL-17A;Cytotoxic T-lymphocyte-associated antigen 8;CTLA-8;IL17A;CTLA8, IL17;

gene full name :

Interleukin-17A

molecular weight :

17504 MW

protein function :

Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

subcellular localization :

Secreted.

tissue specificity :

Restricted to activated memory T-cells.

protein name :

Interleukin-17A

sequence similarities :

Belongs to the IL-17 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 54 276 763 52 257 703 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 CV(%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 62 58 57 3.31 5.8% Sample 2 276 273 283 261 273 7.94 2.9% Sample 3 763 768 854 781 791 36.67 4.6% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.163 0.238 0.383 0.683 1.045 1.663 2.251

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

4/26/19 3:08

more info or order :

Boster product webpage