Human IL-16 PicoKine ELISA Kit | Boster EK0428 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-16 PicoKine ELISA Kit

catalog :

EK0428

quantity :

96 wells/kit, with removable strips.

price :

333 USD

more info or order :

Boster product webpage

citations: 1

Reference
Keseroglu K, Banoglu E, Kizil Y, Aydil U, Gönül I, Yilmaz M, et al. Serum interleukin-16 levels in patients with nasal polyposis. Laryngoscope. 2012;122:961-4 pubmed publisher

image

image 1 :

Human IL-16 PicoKine ELISA Kit standard curve

product information

sku :

EK0428

status :

Enabled

name :

Human IL-16 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL16

price :

333 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL16 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL16 tissue specificity: Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-16. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

Q14005

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: P1204-S1332

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-16 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Interleukin 16(IL-16) is a cytokine that released by a variety of cells(including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor(LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins

synonyms :

Pro-interleukin-16;Interleukin-16;IL-16;Lymphocyte chemoattractant factor;LCF;IL16;

gene full name :

Pro-interleukin-16

molecular weight :

141752 MW

protein function :

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

subcellular localization :

Interleukin-16: Secreted.

tissue specificity :

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

protein name :

Pro-interleukin-16

sequence similarities :

Contains 4 PDZ (DHR) domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV(%) 4.1% 6.8% 5% 4.1% 8.6% 5.7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 64 55 56 58 3.5 6% Sample 2 213 197 219 232 215 12.57 5.8% Sample 3 983 978 906 908 943 36.79 3.9% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.058 0.128 0.249 0.500 0.926 1.673 2.311

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

5/7/19 5:44

more info or order :

Boster product webpage