Human IL-15 PicoKine ELISA Kit | Boster EK0426 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-15 PicoKine ELISA Kit

catalog :

EK0426

quantity :

96 wells/kit, with removable strips.

price :

300 USD

more info or order :

Boster product webpage

citations: 1

Reference
Cao L, Lu Y, Ma M, Xue H, Zhao Y, Yu H, et al. [Levels of serum interleukin-15 and the expression of T-helper lymphocyte subsets in peripheral blood of children with juvenile rheumatoid arthritis]. Zhongguo Dang Dai Er Ke Za Zhi. 2006;8:9-12 pubmed

image

image 1 :

Human IL-15 PicoKine ELISA Kit standard curve

product information

sku :

EK0426

status :

Enabled

name :

Human IL-15 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL15

price :

300 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL15 with <3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL15 tissue specificity: Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-15. 96wells/kit, with removable strips.

description1 :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human IL-15 PicoKine Fast ELISA Kit and many others.

size :

96 wells/kit, with removable strips.

uniprot id :

P40933

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: N49-S162

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<3pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-15 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml

background :

Interleukin(IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2(IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

research category :

Antiviral Signaling, Cancer, Cytokines, Immune System Diseases, Immunology, Innate Immunity, Interleukins, Metabolism, Nk Cells, Obesity, Tumor Immunology

synonyms :

Interleukin-15;IL-15;IL15;

gene full name :

Interleukin-15

molecular weight :

18086 MW

protein function :

Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

subcellular localization :

Isoform IL15-S48AA: Secreted.

tissue specificity :

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

protein name :

Interleukin-15

sequence similarities :

Belongs to the IL-15/IL-21 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV(%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 37 37 35 36 1 2.7% Sample 2 140 151 160 164 153 9.22 6% Sample 3 427 395 364 407 398 22.84 5.7% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage