Mouse IL-13 PicoKine ELISA Kit | Boster EK0425 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IL-13 PicoKine ELISA Kit

catalog :

EK0425

quantity :

96 wells/kit, with removable strips.

price :

430 USD

more info or order :

Boster product webpage

citations: 1

Reference
Jiang Y, Yin K, Jin W, Wu L, Li C. [Experimental study on the Der f 1 mRNA molecules derived from dermatophagoides farinae for specific immunotherapy on murine model of asthma]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2014;32:268-73 pubmed

image

image 1 :

Mouse IL-13 PicoKine ELISA Kit standard curve

product information

sku :

EK0425

status :

Enabled

name :

Mouse IL-13 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL13

price :

430 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse IL13 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL13 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-13. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P20109

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: S26-F131

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IL-13 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Interleukin(IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins

synonyms :

Interleukin-13;IL-13;T-cell activation protein P600;Il13;Il-13;

gene full name :

Interleukin-13

molecular weight :

14108 MW

protein function :

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity).

subcellular localization :

Secreted.

protein name :

Interleukin-13

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 40 209 526 36 193 532 Standard deviation 2.68 15.46 30.5 2.41 16.79 32.45 CV(%) 6.7% 7.4% 5.8% 6.7% 8.7% 6.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 38 36 37 2.23 6% Sample 2 209 191 179 193 193 10.67 5.5% Sample 3 526 501 535 503 516 14.61 2.8% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.116 0.186 0.234 0.358 0.584 1.105 1.714 2.243

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

4/17/19 2:45

more info or order :

Boster product webpage