Human IL-12(p40) PicoKine ELISA Kit | Boster EK0423 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-12(p40) PicoKine ELISA Kit

catalog :

EK0423

quantity :

96 wells/kit, with removable strips.

citations: 1

Reference
Kalt A, Schneider T, Ring S, Hoffmann J, Zeitz M, Stallmach A, et al. Decreased levels of interleukin-12p40 in the serum of patients with Whipple's disease. Int J Colorectal Dis. 2006;21:114-20 pubmed

product information

sku :

EK0423

status :

Enabled

name :

Human IL-12(p40) PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL12B

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Il-12/IL12B with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il-12/IL12B tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-12(p40). 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P29460

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: I23-S328

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is cross-reactivity with IL-12(p70), IL-23(p19/p40)< 5%.

sensitivity :

<2pg/ml

kit components :

2ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-12(p40) PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Interleukin-12(IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40-kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit(p35) is related to IL6 and GCSF. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40(IL12B) to 5q31-q33 and p35(IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage / Inflamm.

synonyms :

Interleukin-12 subunit beta;IL-12B;Cytotoxic lymphocyte maturation factor 40 kDa subunit;CLMF p40;IL-12 subunit p40;NK cell stimulatory factor chain 2;NKSF2;IL12B;NKSF2;

gene full name :

Interleukin-12 subunit beta

molecular weight :

37169 MW

protein function :

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

subcellular localization :

Secreted.

protein name :

Interleukin-12 subunit beta

sequence similarities :

Belongs to the type I cytokine receptor family. Type 3 subfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 75 257 881 75 271 808 Standard deviation 4.5 13.1 63.43 6%5 18.69 71.1 CV(%) 6% 8.8%% 7.2% 7% 6.9% 8.8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 75 66 64 70 5.04 7.2% Sample 2 257 230 241 245 243 9.65 3.9% Sample 3 881 962 909 827 894 48.74 5.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.135 0.218 0.400 0.753 1.253 1.884 2.567

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53