Human IL-12(p70) PicoKine ELISA Kit | Boster EK0421 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-12(p70) PicoKine ELISA Kit

catalog :

EK0421

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 1

Reference
Szaflarska A, Szczepanik A, Siedlar M, Czupryna A, Sierzega M, Popiela T, et al. Preoperative plasma level of IL-10 but not of proinflammatory cytokines is an independent prognostic factor in patients with gastric cancer. Anticancer Res. 2009;29:5005-12 pubmed

image

image 1 :

Human IL-12(p70) PicoKine ELISA Kit standard curve

product information

sku :

EK0421

status :

Enabled

name :

Human IL-12(p70) PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL12A

price :

250 USD

assay range :

7.8pg/ml-500pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Il 12/IL12A with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 12/IL12A tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-12(p70). 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

O60595

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: (I23-S328)+(R23-S219)

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates and serum.

applications :

ELISA

reactivity :

Human

image labels :

Human IL-12(p70) PicoKine ELISA Kit standard curve

assay procedure step 1 :

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

background :

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

research category :

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

synonyms :

Interleukin 12 ;Interleukin 12, P35 ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ;IL12A ;hCG_1685939 ;

gene full name :

interleukin 12A

molecular weight :

28293 MW

protein name :

Interleukin-12 subunit alpha

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 18 51 294 19 55 310 Standard deviation 1.31 3.41 17.38 1.67 3.79 21.39 CV(%) 7.3% 6.7% 5.2% 8.8% 6.9% 6.9%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 20 17 18 1.08 6% Sample 2 51 59 60 54 56 3.67 6.5% Sample 3 294 290 284 296 291 4.58 1.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.019 0.069 0.126 0.239 0.441 0.834 1.441 2.186

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

4/30/19 3:10

more info or order :

Boster product webpage