Human IL-11 PicoKine ELISA Kit | Boster EK0419 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-11 PicoKine ELISA Kit

catalog :

EK0419

quantity :

96 wells/kit, with removable strips.

price :

333 USD

more info or order :

Boster product webpage

citations: 1

Reference
McCloy M, Roberts I, Howarth L, Watts T, Murray N. Interleukin-11 levels in healthy and thrombocytopenic neonates. Pediatr Res. 2002;51:756-60 pubmed

image

image 1 :

Human IL-11 PicoKine ELISA Kit standard curve

product information

sku :

EK0419

status :

Enabled

name :

Human IL-11 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

IL11

price :

333 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL11 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL11 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-11. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P20809

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: P22-L199

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-11 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.

research category :

Adaptive Immunity, Angiogenesis, B Cells, Cancer, Cardiovascular, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Myeloid, Non-Cd, Secreted Molecules, Stem Cells, Tumor Immunology

synonyms :

Interleukin-11;IL-11;Adipogenesis inhibitory factor ;AGIF ;Oprelvekin;IL11;

gene full name :

Interleukin-11

molecular weight :

21429 MW

protein function :

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation (PubMed:12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3.

subcellular localization :

Secreted .

protein name :

Interleukin-11

sequence similarities :

Belongs to the IL-6 superfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 70 290 882 67 281 907 Standard deviation 3.22 20.3 42.33 3.81 23.04 45.35 CV(%) 4.6% 7% 4.8% 5.7% 8.2% 5%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 65 79 74 72 5.14 7.1% Sample 2 290 286 303 269 287 12.14 4.2% Sample 3 882 761 786 893 830 57.81 6.9% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.152 0.231 0.271 0.345 0.507 0.812 1.333 2.186

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/22/19 5:04

more info or order :

Boster product webpage