Human IL-10 PicoKine ELISA Kit | Boster EK0416 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-10 PicoKine ELISA Kit

catalog :

EK0416

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 1

Reference
Chen D, Li Y, Lan S, Pan X, Zhou L, Liang M, et al. [The level and clinical significance of Toll-like receptor 4 in children with severe sepsis]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2011;23:475-7 pubmed

image

image 1 :

Human IL-10 PicoKine ELISA Kit standard curve

product information

sku :

EK0416

status :

Enabled

name :

Human IL-10 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL10

price :

250 USD

assay range :

7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL10 with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL10 tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-10. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P22301

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: S19-N178

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<0.5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-10 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

background :

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

research category :

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

synonyms :

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10;

gene full name :

Interleukin-10

molecular weight :

20517 MW

protein function :

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

subcellular localization :

Secreted.

tissue specificity :

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

protein name :

Interleukin-10

sequence similarities :

Belongs to the IL-10 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 16 53 113 15 54 122 Standard deviation 1.02 2.12 7.34 1.24 3.02 9.88 CV(%) 6.4% 4% 6.5% 8.3% 5.6% 8.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 17 15 15 15 0.82 5.4% Sample 2 53 45 47 48 48 2.94 6.1% Sample 3 113 123 106 104 111 7.43 6.6% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.070 0.151 0.187 0.295 0.538 0.858 1.622 2.281

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse monoclonal antibody from mouse

last modified :

3/7/19 6:53

more info or order :

Boster product webpage