Human IL-8 PicoKine ELISA Kit | Boster EK0413 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-8 PicoKine ELISA Kit

catalog :

EK0413

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 1

Reference
Cojocaru I, Muşuroi C, Iacob S, Cojocaru M. Investigation of TNF-alpha, IL-6, IL-8 and of procalcitonin in patients with neurologic complications in sepsis. Rom J Intern Med. 2003;41:83-93 pubmed

image

image 1 :

Human IL-8 PicoKine ELISA Kit standard curve

product information

sku :

EK0413

status :

Enabled

name :

Human IL-8 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

CXCL8

price :

250 USD

assay range :

15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human CXCL8 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CXCL8 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-8. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P10145

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: S28-S99

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-8 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

research category :

Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment

synonyms :

Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8;(Ala-IL-8)77;GCP/IL-8 protein II;IL-8(1-77);IL8/NAP1 form II;MDNCF-b;IL-8(5-77);IL-8(6-77);(Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8(7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8(8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8(9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8;

gene full name :

Interleukin-8

molecular weight :

11098 MW

protein function :

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

subcellular localization :

Secreted.

protein name :

Interleukin-8

sequence similarities :

Belongs to the intercrine alpha (chemokine CxC) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV(%) 4.5% 4.3% 6.1% 5% 6.1% 7.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.106 0.216 0.385 0.817 1.212 1.818 2.243

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage