Rat IL-6 PicoKine ELISA Kit | Boster EK0412 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat IL-6 PicoKine ELISA Kit

catalog :

EK0412

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
He G, Zhou K, Zhang R, Wang Y, Chen X. Impact of intestinal ischemia/reperfusion and lymph drainage on distant organs in rats. World J Gastroenterol. 2012;18:7271-8 pubmed publisher

image

image 1 :

Rat IL-6 PicoKine ELISA Kit standard curve

product information

sku :

EK0412

status :

Enabled

name :

Rat IL-6 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL6

price :

380 USD

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Rat IL6 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-6. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P20607

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: F25-T211

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Rat

image labels :

Rat IL-6 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

research category :

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

synonyms :

Interleukin-6;IL-6;Il6;Il-6;

gene full name :

Interleukin-6

molecular weight :

24357 MW

protein function :

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).

subcellular localization :

Secreted.

protein name :

Interleukin-6

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 193 579 1531 210 564 1466 Standard deviation 9.65 35.89 90.32 11.55 40.04 109.95 CV(%) 5% 6.2% 5.9% 5.5% 7.1% 7.5%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 192 175 177 184 8.28 4.5% Sample 2 579 631 610 577 599 22.52 3.7% Sample 3 1531 1470 1340 1471 1453 69.76 4.8% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.183 0.190 0.264 0.352 0.530 0.811 1.140 1.820

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage