Mouse IL-6 PicoKine ELISA Kit | Boster EK0411 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IL-6 PicoKine ELISA Kit

catalog :

EK0411

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 2

Reference
Zhang Y, Feng J, Cui J, Yang G, Zhu X. Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Mol Med Rep. 2018;17:5805-5813 pubmed publisher
Huang L, Wang M, Cheng Z, Xue P, Jin T, Yang X, et al. Effects of Chai-Qin-Cheng-Qi decoction () on acute pancreatitis-associated lung injury in mice with acute necrotizing pancreatitis. Chin J Integr Med. 2012;: pubmed

image

image 1 :

Mouse IL-6 PicoKine ELISA Kit standard curve

product information

sku :

EK0411

status :

Enabled

name :

Mouse IL-6 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL6

price :

320 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse IL6 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-6. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

A2RTD1

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: F25-T211

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IL-6 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

research category :

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

synonyms :

Interleukin 6 ;Interleukin 6, isoform CRA_b ;Il6 ;mCG_11634 ;

gene full name :

interleukin 6

molecular weight :

24384 MW

protein name :

Interleukin-6

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 36 159 436 36 147 482 Standard deviation 2.41 7.79 34.94 2.91 7.93 40.48 CV(%) 6.7% 4.9% 7.2% 8.1% 5.4% 8.4%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 39 39 34 37 2.12 5.7% Sample 2 159 147 154 170 157 8.38 5.3% Sample 3 495 443 464 434 459 23.46 5.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.085 0.141 0.237 0.452 0.816 1.470 2.281

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage