Human IL-6 PicoKine ELISA Kit | Boster EK0410 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-6 PicoKine ELISA Kit

catalog :

EK0410

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 2

Reference
Wang S, Li J, Tian J, Yu Z, Gao K, Shao J, et al. High amplitude and low frequency cyclic mechanical strain promotes degeneration of human nucleus pulposus cells via the NF-?B p65 pathway. J Cell Physiol. 2018;233:7206-7216 pubmed publisher
Song X, Chen X, Zhang W, Li X, Hei G, Li Y, et al. [Study of adiponectin, IL-1β, IL-6 and TNF-α in first episode drug naїve schizophrenia]. Zhonghua Yi Xue Za Zhi. 2013;93:3256-60 pubmed

image

image 1 :

Human IL-6 PicoKine ELISA Kit standard curve

product information

sku :

EK0410

status :

Enabled

name :

Human IL-6 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL6

price :

250 USD

assay range :

4.69pg/ml-300pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL6 with <0.3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P05231

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: P29-M212

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<0.3pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-6 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.75pg/ml, 9.38pg/ml, 4.69pg/ml

background :

The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

research category :

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

synonyms :

Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2

gene full name :

interleukin 6

molecular weight :

23,718 MW

protein function :

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

protein name :

Interleukin-6

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 10 38 145 9 37 130 Standard deviation 0.62 1.67 10.73 0.64 1.66 11.18 CV(%) 6.2% 4.4% 7.4% 7.2% 4.5% 8.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 10 12 11 10 10 0.82 8.2% Sample 2 38 43 40 42 40 1.92 4.8% Sample 3 145 149 145 150 147 2.27 1.5% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.039 0.107 0.186 0.327 0.616 1.062 1.923 2.420

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

6/5/19 0:54

more info or order :

Boster product webpage