Mouse IL-5 PicoKine ELISA Kit | Boster EK0408 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IL-5 PicoKine ELISA Kit

catalog :

EK0408

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Ma J, Xia J, Cheng X, Wang C. 1,25-dihydroxyvitamin D₃ pretreatment enhances the efficacy of allergen immunotherapy in a mouse allergic asthma model. Chin Med J (Engl). 2010;123:3591-6 pubmed

image

image 1 :

Mouse IL-5 PicoKine ELISA Kit standard curve

product information

sku :

EK0408

status :

Enabled

name :

Mouse IL-5 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL5

price :

320 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse IL5 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL5 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-5. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P04401

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: M21-G133

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates and serum.

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IL-5 PicoKine ELISA Kit standard curve

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

research category :

Adaptive Immunity, B Cells, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Non-Cd, Tumor Immunology

synonyms :

Interleukin-5;IL-5;B-cell growth factor II;BCGF-II;Cytotoxic T-lymphocyte inducer;Eosinophil differentiation factor;T-cell replacing factor;TRF;Il5;Il-5;

gene full name :

Interleukin-5

molecular weight :

15410 MW

protein function :

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

subcellular localization :

Secreted.

protein name :

Interleukin-5

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV(%) 7% 4.2% 4.7% 7.6% 5% 6.4%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 37 35 34 2.27 6.6% Sample 2 220 228 267 256 242 19.35 7.9% Sample 3 453 402 397 434 421 23.07 5.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.083 0.144 0.258 0.467 0.882 1.754 2.352

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage