Human IL-5 PicoKine ELISA Kit | Boster EK0407 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-5 PicoKine ELISA Kit

catalog :

EK0407

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Liao P, Wu S. [Serum levels of IL-5 and LTB4 in children with Henoch-Schonlein purpura]. Zhongguo Dang Dai Er Ke Za Zhi. 2006;8:198-200 pubmed

image

image 1 :

Human IL-5 PicoKine ELISA Kit standard curve

product information

sku :

EK0407

status :

Enabled

name :

Human IL-5 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL5

price :

320 USD

assay range :

7.8pg/ml-500pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL5 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL5 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-5. 96wells/kit, with removable strips.

description1 :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human IL-5 PicoKine Fast ELISA Kit and many others.

size :

96 wells/kit, with removable strips.

uniprot id :

P05113

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: I20-S134

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum, plasma (heparin, EDTA) and urine.

applications :

ELISA

reactivity :

Human

image labels :

Human IL-5 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

background :

Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

research category :

Adaptive Immunity, B Cells, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Non-Cd, Tumor Immunology

synonyms :

Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5;

gene full name :

Interleukin-5

molecular weight :

15238 MW

protein function :

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

subcellular localization :

Secreted.

protein name :

Interleukin-5

sequence similarities :

Belongs to the IL-5 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 9 73 238 8 70 234 Standard deviation 0.7 4.67 16.42 0.74 4.55 20.12 CV(%) 7.8% 6.4% 6.9% 9.3% 6.5% 8.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 10 10 9 9 0.5 5.5% Sample 2 73 73 74 62 70 4.92 7% Sample 3 238 246 219 235 234 9.81 4.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.005 0.078 0.150 0.269 0.501 0.862 1.545 2.201

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage