Rat IL-4 PicoKine ELISA Kit | Boster EK0406 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat IL-4 PicoKine ELISA Kit

catalog :

EK0406

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Li C, Ye L, Chen X, Li S, Cai X, Dong L, et al. [Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone]. Zhonghua Er Ke Za Zhi. 2005;43:521-5 pubmed

image

image 1 :

Rat IL-4 PicoKine ELISA Kit standard curve

product information

sku :

EK0406

status :

Enabled

name :

Rat IL-4 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL4

price :

320 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Rat IL4 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL4 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-4. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P20096

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: C25-S147

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates and serum.

applications :

ELISA

reactivity :

Rat

image labels :

Rat IL-4 PicoKine ELISA Kit standard curve

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.

research category :

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

synonyms :

Interleukin-4;IL-4;B-cell IGG differentiation factor;B-cell growth factor 1;B-cell stimulatory factor 1;BSF-1;Lymphocyte stimulatory factor 1;Il4;Il-4;

gene full name :

Interleukin-4

molecular weight :

16247 MW

protein function :

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

subcellular localization :

Secreted.

protein name :

Interleukin-4

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 35 164 456 32 175 481 Standard deviation 2.45 10.66 21.43 2.4 11.37 28.37 CV(%) 7% 6.5% 4.7% 7.5% 6.5% 5.9%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 30 36 34 2.34 6.8% Sample 2 164 156 144 155 154 7.11 4.6% Sample 3 456 463 512 430 465 29.65 6.3% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.107 0.143 0.229 0.470 0.793 1.561 2.163

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage