ELISA/assay

Mouse IL-4 PicoKine ELISA Kit

EK0405

96 wells/kit, with removable strips.

320 USD

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Mouse IL-4 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

IL4

320 USD

7.8pg/ml-500pg/ml

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Mouse IL4 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL4 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-4. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P07750

No

Expression system for standard: E.coli; Immunogen sequence: H21-S140

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates and serum.

ELISA

Mouse

Mouse IL-4 PicoKine ELISA Kit standard curve

500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Interleukin-4;IL-4;B-cell IgG differentiation factor;B-cell growth factor 1;B-cell stimulatory factor 1;BSF-1;IGG1 induction factor;Lymphocyte stimulatory factor 1;Il4;Il-4;

Interleukin-4

15834 MW

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Secreted.

Interleukin-4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 15 96 280 16 93 275 Standard deviation 1.11 6.62 20.44 1.48 6.69 20.9 CV(%) 7.4% 6.9% 7.3% 9.3% 7.2% 7.6%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 14 13 16 14 1.11 7.9% Sample 2 96 90 93 94 93 2.16 2.3% Sample 3 280 285 256 248 267 15.61 5.8% *number of samples for each test n=16.

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.056 0.097 0.131 0.212 0.374 0.813 1.489 2.284

15-25min

monoclonal antibody from rat polyclonal antibody from goat

5/29/19 3:23