ELISA/assay

Human IL-4 PicoKine ELISA Kit

EK0404

96 wells/kit, with removable strips.

250 USD

Enabled

Human IL-4 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

IL4

250 USD

15.6pg/ml-1000pg/ml

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human IL4 with <1.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL4 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-4. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

D4HNR6

No

Expression system for standard: E.coli; Immunogen sequence: H25-S153

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1.5pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

Human IL-4 PicoKine ELISA Kit standard curve

heparin or EDTA

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Interleukin-4 ;IL-4 ;B-cell stimulatory factor 1 ;Lymphocyte stimulatory factor 1 ;IL4 ;hCG_24113 ;

Interleukin-4

17492 MW

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Secreted .

Interleukin-4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV(%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 34 34 33 1.5 4.5% Sample 2 216 213 203 197 207 7.62 3.6% Sample 3 508 515 448 502 493 26.52 5.3% *number of samples for each test n=16.

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.042 0.067 0.129 0.339 0.780 1.651 2.241

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

3/7/19 6:53