Human IL-3 PicoKine ELISA Kit | Boster EK0402 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-3 PicoKine ELISA Kit

catalog :

EK0402

quantity :

96 wells/kit, with removable strips.

price :

320 USD

more info or order :

Boster product webpage

citations: 1

Reference
Hellmich B, Ciaglo A, Schatz H, Coakley G. Autoantibodies against granulocyte-macrophage colony stimulating factor and interleukin-3 are rare in patients with Felty's syndrome. Ann Rheum Dis. 2004;63:862-6 pubmed

image

image 1 :

Human IL-3 PicoKine ELISA Kit standard curve

product information

sku :

EK0402

status :

Enabled

name :

Human IL-3 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL3

price :

320 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL3 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL3 tissue specificity: Activated T-cells, mast cells, natural killer cells.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-3. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P08700

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A20-F152

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-3 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins

synonyms :

Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;IL3;

gene full name :

Interleukin-3

molecular weight :

17233 MW

protein function :

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

subcellular localization :

Secreted.

tissue specificity :

Activated T-cells, mast cells, natural killer cells.

protein name :

Interleukin-3

sequence similarities :

Belongs to the IL-3 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 33 205 387 31 190 374 Standard deviation 1.68 9.63 21.736 1.73 8.93 28.42 CV(%) 7.6% 4.7% 7.2% 5.6% 4.7% 7.6%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 33 37 35 2 5.7% Sample 2 205 240 222 228 223 12.61 5.6% Sample 3 387 460 404 393 411 28.93 7% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.111 0.135 0.212 0.351 0.633 1.155 1.885

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/13/19 5:47

more info or order :

Boster product webpage