Rat IL-2 PicoKine ELISA Kit | Boster EK0399 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat IL-2 PicoKine ELISA Kit

catalog :

EK0399

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 1

Reference
Oh J, Kim M, Shin M, Lee H, Lee J, Wee W. The anti-inflammatory effect of subconjunctival bevacizumab on chemically burned rat corneas. Curr Eye Res. 2009;34:85-91 pubmed publisher

image

image 1 :

Rat IL-2 PicoKine ELISA Kit standard curve

product information

sku :

EK0399

status :

Enabled

name :

Rat IL-2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL2

price :

250 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Rat IL2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL2 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P17108

host :

immunogen :

Expression system for standard: E.coli,A21-Q155

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Rat

image labels :

Rat IL-2 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA, citrate

assay procedure step 1 :

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4.

research category :

Adaptive Immunity, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells

synonyms :

Interleukin-2;IL-2;T-cell growth factor;TCGF;Il2;Il-2;

gene full name :

Interleukin-2

molecular weight :

17633 MW

protein function :

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

subcellular localization :

Secreted.

protein name :

Interleukin-2

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 62 243 1041 56 254 1022 Standard deviation 4.71 15.06 53.09 4.81 17.52 71.54 CV(%) 7.6% 6.2% 7%% 8.6% 6.9% 7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 61 60 63 61 1.11 1.8% Sample 2 243 247 277 267 258 14.02 5.4% Sample 3 1041 992 897 885 953 65.23 6.8% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000 O.D. 0.024 0.096 0.173 0.339 0.764 1.094 1.696 2.295

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage