Human IL-2 PicoKine ELISA Kit | Boster EK0397 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-2 PicoKine ELISA Kit

catalog :

EK0397

quantity :

96 wells/kit, with removable strips.

price :

250 USD

more info or order :

Boster product webpage

citations: 1

Reference
Chen J, Zhou Y, Jin X, Chen S. Expression of interleukin-2 in Candidal balanoposthitis and its clinical significance. Chin Med J (Engl). 2011;124:2776-8 pubmed

image

image 1 :

Human IL-2 PicoKine ELISA Kit standard curve

product information

sku :

EK0397

status :

Enabled

name :

Human IL-2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL2

price :

250 USD

assay range :

15.6pg/ml-1000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL2 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P60568

host :

immunogen :

Expression system for standard: E.coli, Immunogen sequence: A21-T153

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-2 PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

background :

Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

research category :

Adaptive Immunity, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells

synonyms :

Interleukin-2;IL-2;T-cell growth factor;TCGF;Aldesleukin;IL2;

gene full name :

Interleukin-2

molecular weight :

17628 MW

protein function :

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

subcellular localization :

Secreted.

protein name :

Interleukin-2

sequence similarities :

Belongs to the IL-2 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV(%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.123 0.212 0.336 0.609 1.031 1.798 2.197

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage