Human IL1R2/Il 1 Rii PicoKine ELISA Kit | Boster EK0396 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL1R2/Il 1 Rii PicoKine ELISA Kit

catalog :

EK0396

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 1

Reference
Hagiwara K, Kitajima K, Yamanaka H, Kirisawa R, Iwai H. Development of a sandwich ELISA assay for measuring bovine soluble type II IL-1 receptor (IL1R2) concentration in serum and milk. Cytokine. 2005;32:132-6 pubmed

image

image 1 :

Human IL1R2 PicoKine ELISA Kit standard curve

product information

sku :

EK0396

status :

Enabled

name :

Human IL1R2/Il 1 Rii PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

IL1R2

price :

369 USD

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human Il-1 Rii/IL1R2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il-1 Rii/IL1R2 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1R2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P27930

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: F14-E343

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates and serum.

applications :

ELISA

reactivity :

Human

image labels :

Human IL1R2 PicoKine ELISA Kit standard curve

assay procedure step 1 :

2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Interleukin 1 receptor, type II(IL1R2) also known as CD121b(Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta(IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

research category :

Lymphoid, Stem Cells

synonyms :

Interleukin-1 receptor type 2;IL-1R-2;IL-1RT-2;IL-1RT2;CD121 antigen-like family member B;CDw121b;IL-1 type II receptor;Interleukin-1 receptor beta;IL-1R-beta;Interleukin-1 receptor type II;CD121b;Interleukin-1 receptor type 2, membrane form;mIL-1R2;mIL-1RII;Interleukin-1 receptor type 2, soluble form;sIL-1R2;sIL-1RII;IL1R2;IL1RB;

gene full name :

Interleukin-1 receptor type 2

molecular weight :

45421 MW

protein function :

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

subcellular localization :

Isoform Short: Secreted .

protein name :

Interleukin-1 receptor type 2

sequence similarities :

Belongs to the interleukin-1 receptor family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 100 261 838 99 279 861 Standard deviation 7.9 14.61 42.73 7.92 17.85 57.68 CV(%) 7.9% 5.6% 6.7%% 8% 6.4% 6.7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 94 89 86 92 5.3 5.7% Sample 2 261 291 293 274 279 13.1 4.6% Sample 3 838 804 847 848 834 17.89 2.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000 O.D. 0.066 0.088 0.144 0.254 0.544 1.007 1.821 2.388

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage