Mouse IL-1 Beta PicoKine ELISA Kit | Boster EK0394 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IL-1 Beta PicoKine ELISA Kit

catalog :

EK0394

quantity :

96 wells/kit, with removable strips.

price :

420 USD

more info or order :

Boster product webpage

citations: 3

Reference
Huang X, Feng Z, Jiang Y, Li J, Xiang Q, Guo S, et al. VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages. Sci Adv. 2019;5:eaau7426 pubmed publisher
Zhang Y, Feng J, Cui J, Yang G, Zhu X. Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Mol Med Rep. 2018;17:5805-5813 pubmed publisher
Oh H, Jang J, Li Z, Park S, Yoon K. Effects of umbilical cord serum eye drops in a mouse model of ocular chemical burn. Curr Eye Res. 2012;37:1084-90 pubmed publisher

image

image 1 :

Mouse IL-1 beta PicoKine ELISA Kit standard curve

product information

sku :

EK0394

status :

Enabled

name :

Mouse IL-1 Beta PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL1B

price :

420 USD

assay range :

12.5pg/ml-800pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse Il 1 Beta/IL1B with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 1 Beta/IL1B tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 beta. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P10749

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: V118-S269

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IL-1 beta PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

800pg/ml, 400pg/ml, 200pg/ml, 100pg/ml, 50pg/ml, 25pg/ml, 12.5pg/ml

background :

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

research category :

Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation

synonyms :

Interleukin-1 beta;IL-1 beta;Il1b;

gene full name :

Interleukin-1 beta

molecular weight :

30931 MW

protein function :

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

subcellular localization :

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

protein name :

Interleukin-1 beta

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV(%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.123 0.212 0.336 0.609 1.031 1.798 2.197

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

6/10/19 7:38

more info or order :

Boster product webpage