Human IL-1 Alpha PicoKine ELISA Kit | Boster EK0389 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-1 Alpha PicoKine ELISA Kit

catalog :

EK0389

quantity :

96 wells/kit, with removable strips.

price :

260 USD

more info or order :

Boster product webpage

citations: 1

Reference
Mardanian F, Sheikh Soleimani Z. The diagnostic role of cervico-vaginal fluid interleukins-1α in endometriosis: A case-control study. J Res Med Sci. 2014;19:1145-9 pubmed

image

image 1 :

Human IL-1 alpha PicoKine ELISA Kit standard curve

product information

sku :

EK0389

status :

Enabled

name :

Human IL-1 Alpha PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

IL1A

price :

260 USD

assay range :

4.7pg/ml-300pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IL1A with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL1A tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 alpha. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P01583

host :

immunogen :

Expression system for standard: E.coli, Immunogen sequence: S113-A271

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<0.5pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human IL-1 alpha PicoKine ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.8pg/ml, 9.4pg/ml, 4.7pg/ml

background :

Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.

research category :

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

synonyms :

Interleukin-1 alpha;IL-1 alpha;Hematopoietin-1;IL1A;IL1F1;

gene full name :

Interleukin-1 alpha

molecular weight :

30607 MW

protein function :

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

subcellular localization :

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

protein name :

Interleukin-1 alpha

sequence similarities :

Belongs to the IL-1 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV(%) 7% 4.8% 4.4% 7.1% 4.9% 5.9%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.133

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage